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Tris base is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Basic Information | |
Product name |
Tris base |
Synonyms |
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane, Trometamol |
CAS |
77-86-1 |
Molecular Formula |
C4H11NO3 |
Molecular weight |
121.14 |
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Hazard Codes |
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Risk Statements |
; |
Safety Statements |
S26;S37/39; |
Xi:Irritant| Application |
Trometamol has been used:
• as a component of H buffer (cell dissociation buffer)
• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique.
• as an assay buffer for reconstitution of extracted and dried protein samples.
• to prepare Tris-HCl buffer that is used to stabilize proteins.
• as a buffer to extract carotenoid from tubers.
• as a component of sample buffer during protein extraction prior to western blotting[12]
• as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay.
• as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate.
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| Company introduction |
Our company has many years of rich experience in producing tris base Trometamol THAM buffer 77-86-1 so that the quality can be guaranteed, and you can buy with confidence. Our high-quality service system and good reputation have been unanimously recognized by customers. With excellent product quality and good after-sales service, our company has established a sincere and trustworthy corporate image.
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https://www.hbynm.com/